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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become noticed in many tumors, which results in chromosome instability, and thus may well lead to tumorigenesis. This protein was identified to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a important position in advertising and marketing mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually explained. Two extra splice variants had been claimed but in-depth nucleotide sequence facts just isn't yet available.
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Blocking peptides are peptides that bind especially to your target antibody and block antibody binding. These peptide normally contains the epitope acknowledged because of the antibody.
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This tutorial outlines even further achievable optimization of cellular and immunoassay parameters to be certain the very best final results are...
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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to ensure the best possible results are...
Antibodies bound to the blocking peptide now not bind into the epitope about the focus on protein. By comparing the staining from your blocked antibody versus the antibody alone, you can see which staining is unique.
Concentrate on Facts Cyclin E belongs on the hugely conserved cyclin loved ones, whose associates are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic event. Cyclin E varieties a fancy with and capabilities for a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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